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guide rna expression cassette  (Addgene inc)


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    Addgene inc guide rna expression cassette
    Guide Rna Expression Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 3781 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guide rna expression cassette/product/Addgene inc
    Average 96 stars, based on 3781 article reviews
    guide rna expression cassette - by Bioz Stars, 2026-02
    96/100 stars

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    The pgRNA-YFP vectors are used to screen the effectiveness of gRNAs in Agrobacterium -mediated transient assays. Transient expression of pgRNA-YFP alone in N. benthamiana does not produce any fluorescent signals. (A) Co-expression of p NbEDS1 gRNA-YFP with pCas9- NbNDR/NbEDS1 gRNA restored the YFP signals in N. benthamiana . (B) Co-expression of p NbNDR1 gRNA-YFP with pCas9- NbNDR1/NbEDS1 gRNA failed to restore the YFP signals. (C) Co-expression of p NbWRKY70 gRNA-YFP with pCas9- NbWRKY70 gRNA restored the YFP signals. (D, E) Co-expression of either p GmKTI1 gRNA-YFP or p GmKTI3 gRNA-YFP with pCas9- GmKTI1/GmKTI3 gRNA restored the YFP signals in N. benthamiana . Scale bar represents 100 µm.

    Journal: Frontiers in Plant Science

    Article Title: Evaluate the guide RNA effectiveness via Agrobacterium -mediated transient assays in Nicotiana benthamiana

    doi: 10.3389/fpls.2023.1111683

    Figure Lengend Snippet: The pgRNA-YFP vectors are used to screen the effectiveness of gRNAs in Agrobacterium -mediated transient assays. Transient expression of pgRNA-YFP alone in N. benthamiana does not produce any fluorescent signals. (A) Co-expression of p NbEDS1 gRNA-YFP with pCas9- NbNDR/NbEDS1 gRNA restored the YFP signals in N. benthamiana . (B) Co-expression of p NbNDR1 gRNA-YFP with pCas9- NbNDR1/NbEDS1 gRNA failed to restore the YFP signals. (C) Co-expression of p NbWRKY70 gRNA-YFP with pCas9- NbWRKY70 gRNA restored the YFP signals. (D, E) Co-expression of either p GmKTI1 gRNA-YFP or p GmKTI3 gRNA-YFP with pCas9- GmKTI1/GmKTI3 gRNA restored the YFP signals in N. benthamiana . Scale bar represents 100 µm.

    Article Snippet: The guiding RNA expression cassettes targeting GmKTI1 and GmKTI3 , NbEDS1 and NbNDR1 , and NbWRKY70, respectively , were synthesized at GenScript Biotech Corp.

    Techniques: Expressing

    Sanger sequencing chromatograms display the DNA sequence of GmKTI1 and GmKTI3 that is targeted for gene editing. The open reading frames of GmKTI1 and GmKTI3 in the pCas9-GmKTI1/GmKTI3 gRNA T0 transgenic soybean plants were amplified and sequenced. The double peaks in the sequencing chromatograms indicate the presence of both wild-type and mutant allele of sequences GmKTI1 (A) and GmKTI3 (B) . The wild-type gene sequences are shown on the top of the sequencing chromatograms. The PAM sites are underlined.

    Journal: Frontiers in Plant Science

    Article Title: Evaluate the guide RNA effectiveness via Agrobacterium -mediated transient assays in Nicotiana benthamiana

    doi: 10.3389/fpls.2023.1111683

    Figure Lengend Snippet: Sanger sequencing chromatograms display the DNA sequence of GmKTI1 and GmKTI3 that is targeted for gene editing. The open reading frames of GmKTI1 and GmKTI3 in the pCas9-GmKTI1/GmKTI3 gRNA T0 transgenic soybean plants were amplified and sequenced. The double peaks in the sequencing chromatograms indicate the presence of both wild-type and mutant allele of sequences GmKTI1 (A) and GmKTI3 (B) . The wild-type gene sequences are shown on the top of the sequencing chromatograms. The PAM sites are underlined.

    Article Snippet: The guiding RNA expression cassettes targeting GmKTI1 and GmKTI3 , NbEDS1 and NbNDR1 , and NbWRKY70, respectively , were synthesized at GenScript Biotech Corp.

    Techniques: Sequencing, Transgenic Assay, Amplification, Mutagenesis